Murasighe and Skoog Media Preparation

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Liter
1X
0.5X
0.1X
(check one)
MS Salts*
4.3 g
2.15 g
0.43 g
MES**
0.50 g
0.50 g
0.50 g
ddH20
800ml
800ml
800ml



- Mix, pH to 5.7+ 0.1 with 1N KOH,
- Complete volume to 1L with ddH2O,
- Split into 500 ml flasks for autoclaving.

Options:

Agar: 0%
, 1% (5g/500 ml) , 0.8% (4g/500 ml) , other:
Sucrose: 0%
, 1% (5g/500 ml) , 2% (10g/500 ml) , other:

Other Compounds:


Added Before Autoclaving Added After Autoclaving


- Autoclave 20-25 min, cool MS/agar to 65o before cooling (or adding filter sterilized compounds).
- Label plates:

- Label Sleeve with date, initials.

Notes:

Name: Date:

*MS Salts (Gibco BRL #1117-058), **MES (Sigma M-2933)