| Liter |
1X
|
0.5X
|
0.1X
|
|
(check one)
|
|
|
|
| MS Salts* |
4.3 g
|
2.15 g
|
0.43 g
|
| MES** |
0.50 g
|
0.50 g
|
0.50 g
|
| ddH20 |
800ml
|
800ml
|
800ml
|
- Mix, pH to 5.7+ 0.1 with 1N KOH,
- Complete volume to 1L with ddH2O,
- Split into 500 ml flasks for autoclaving.
Options:
Agar: 0% , 1% (5g/500 ml) , 0.8% (4g/500 ml) , other:
Sucrose: 0% , 1% (5g/500 ml) , 2% (10g/500 ml) , other:
Other Compounds:
Added Before Autoclaving Added After Autoclaving
- Autoclave 20-25 min, cool MS/agar to 65o before cooling (or adding filter sterilized compounds).
- Label plates:
- Label Sleeve with date, initials.
Notes:
Name: Date:
*MS Salts (Gibco BRL #1117-058), **MES (Sigma M-2933)